The impact of Na2O on the synthesis of α-C2SH with different mineral composition and the stability of intermediate and final products

In this work the effect of Na₂O on the synthesis of α-C₂S hydrate (2CaO·SiO₂·H₂O), named as α-C₂SH, with different mineral composition and the stability of intermediate and final products were investigated. It is worth noting that new results was discovered by evaluating the mineral composition of hydrothermally synthesized α-C₂SH samples (200?°C; 2–72?h). It was found that Na₂O additive significantly influenced the formation and stability of intermediate and final products: within 12?h of hydrothermal treatment together with α-C₂SH a new calcium silicate hydrate phase, killalaite, was obtained in the products. Besides, the mineral composition of formed compounds slightly varied by prolonging the duration of synthesis to 24?h. For the first time it was proved that Na⁺ ions are not incorporated into the crystal structure of the main synthesis product, α-C₂SH. On the contrary, the mentioned ions are intercalated into the crystal structure of other calcium silicate hydrates. Moreover, the investigated alkaline additive reduced the recrystallization temperature of the synthesis products to wollastonite by 50?°C and significantly decreased the values of both specific area and total pore volume of α-C₂SH till 8.496?m²/g and 0.05084?cm³/g, respectively. The synthetic products were characterized by XRD, DSC, SEM/EDX and N₂ adsorption analysis.     

Simulations of the Ladle Teeming Process and Verification With Pilot Experiment

The ladle teeming process was investigated by 2D axis‐symmetrical mathematical models and a pilot‐plant experiment. Different turbulence models, including the low Reynolds number k–ε model and the realizable k–ε model both with an enhanced wall treatment (EWT) and a standard wall function (SWF), were used to simulate this process. All of these turbulence model predictions generally agreed well with the experimental results. The velocity distributions in the nozzle were also predicted by these turbulence models. At the late stage of the teeming process, the drain sink flow phenomenon was studied. The combination of an inclined ladle bottom and a gradually expanding nozzle was found to be an effective way to alleviate a drain sink flow.     

Numerical Investigation of Natural Convection in Heterogeneous Rectangular Enclosures

The aim of this study is to investigate numerically the steady natural convection resulting from horizontally heating a rectangular enclosure filled with a fluid and containing uniformly distributed, conducting, fixed and disconnected solid blocks (i.e., not touching each other). In particular, the effects of solid volume fraction, solid–fluid thermal conductivity ratio, and total number of blocks on the heat transfer process are determined by solving the mass, momentum, and energy conservation equations using the finite-volume method. The enclosure aspect ratio is varied from 0.25 to 4, the Rayleigh number from 10⁵ to 10⁸, and the Prandtl number is set as unity. Results of the numerical simulations are presented in terms of the surface-averaged Nusselt number, streamfunction, and streamline and isotherm distributions. The interference phenomenon caused by the solid blocks on the natural convection process is considered in detail and used to explain and predict the surprising and complex behavior of surface-averaged Nusselt number.     

Effect of Fluid Bypassing on the Experimentally Obtained Darcy and Non-Darcy Permeability Parameters of Ceramic Foam Filters

Metallurgical and Materials Transactions B - Ceramic foam filters (CFFs) are used to remove solid particles and inclusions from molten metal. In general, molten metal which is poured on the top of...     

Phenotypically divergent classification of preweaned heifer calves for feed efficiency indexes and their correlations with heat production and thermography

The aims of this study were (1) to assess if there is phenotypical divergence for feed efficiency (FE) during the preweaning phase; (2) if FE is correlated with heat production (HP) measured by the face mask method or (3) by surface skin temperature via thermography, and (4) whether these methods are applicable to preweaned calves. Holstein × Gyr heifer calves (n = 36, birth body weight = 32.4 ± 6.6 kg) were enrolled and on trial between 4 and 12 wk of age and were classified into 2 residual feed intake (RFI) and residual body weight gain (RG) groups: high efficiency (HE; RFI, n = 10; and RG, n = 9) and low efficiency (LE; RFI, n = 10; and RG, n = 8). Calves were fed milk (6 L/d) and solid feed (95% starter and 5% chopped Tifton 85 hay, as fed). Growth was monitored weekly and feed intake (milk and solid feed) daily, during the whole period. Gas exchanges (O₂ consumption and production of CO₂ and CH₄) were obtained using a face mask at 45 ± 5 d of age and HP was estimated. Maximum temperatures were measured at 7 sites with an infrared camera at 62 ± 7 d of age. There was divergence in RFI and RG. Respectively, HE and LE calves had RFI of ?0.14 and 0.13 kg/d, and RG of 0.05 and ?0.07 kg/d. Dry matter intake was 15% lower in HE-RFI compared with LE-RFI, but no differences were observed in average daily weight gain. Within the RG test, no differences were observed in dry matter intake or average daily gain. The HE-RFI calves consumed less O₂ (L/d) and produced less CO₂ (L/d). Heart rate and HP were lower for HE-RFI calves compared with LE-RFI. Residual feed intake was correlated with HP (r = 0.48), O₂ consumption (r = 0.48), CO₂ production (r = 0.48), and heart rate (r = 0.40). No differences were observed in HP and gas exchanges between RG groups. Methane production was null in both groups. Eye temperature measured by thermography was 0.5°C greater in HE-RG than LE-RG calves. Differences in skin temperature between HE and LE calves were not observed at the other sites. These results support the hypothesis that calves are divergent for RFI, RG, and FE during preweaning and divergence tests are applicable during this phase. The face mask method described here is a useful tool for estimating differences in HP among phenotypically divergent RFI calves. Eye temperature measured by infrared thermography may have potential to screen phenotypically divergent RG calves.     

A Study on the Nonmetallic Inclusion Motions in a Swirling Flow Submerged Entry Nozzle in a New Cylindrical Tundish Design

Metallurgical and Materials Transactions B - Different sizes and shapes of nonmetallic inclusions in a swirling flow submerged entry nozzle (SEN) placed in a new tundish design were investigated by...     

Effects of the macroalga Asparagopsis taxiformis and oregano leaves on methane emission,rumen fermentation,and lactational performance of dairy cows

Asparagopsis taxiformis (AT) is a source of multiple halogenated compounds and, in a limited number of studies, has been shown to decrease enteric CH₄ emission in vitro and in vivo. Similarly, oregano has been suggested as a potential CH₄ mitigating agent. This study consisted of 2 in vitro and 2 in vivo experiments. Experiment (Exp.) 1 was aimed at establishing the effect of AT on CH₄ emission in vitro. Two experiments (Exp. 2 and 3) with lactating dairy cows were conducted to determine the antimethanogenic effect of AT and oregano (Exp. 3) in vivo. Another experiment (Exp. 4) was designed to investigate stability of bromoform (CHBr₃) in AT over time. In Exp. 3, 20 Holstein cows were used in a replicated 4 × 4 Latin square design with four 28-d periods. Treatments were basal diet (control) or basal diet supplemented with (dry matter basis) 0.25% AT (LowAT), 0.50% AT (HighAT), or 1.77% oregano (Origanum vulgare L.) leaves. Enteric gas emissions were measured using the GreenFeed system (C-Lock Inc., Rapid City, SD), and rumen samples were collected for fermentation analysis using the ororuminal technique. In Exp.1 (in vitro), relative to the control, AT (at 1% dry matter basis, inclusion rate) decreased CH₄ yield by 98%. In Exp. 3, HighAT decreased average daily CH₄ emission and CH₄ yield by 65% and 55%, respectively, in experimental periods 1 and 2, but had no effect in periods 3 and 4. The differential response to AT among experimental periods was likely a result of a decrease in CHBr₃ concentration in AT over time, as observed in Exp. 4 (up to 84% decrease in 4 mo of storage). In Exp. 3, H₂ emission was increased by AT and, as expected, the proportion of acetate in the total volatile fatty acids in the rumen was decreased and those of propionate and butyrate were increased by HighAT compared with the control. Compared with the control, HighAT decreased dry matter intake, milk yield, and energy-corrected milk yield in Exp. 3. Milk composition was not affected by treatment, except lactose percentage and yield were decreased by HighAT. Concentrations of iodine and bromide in milk were increased by HighAT compared with the control. Milk CHBr₃ concentration and its organoleptic characteristics were not different between control and HighAT. Oregano had no effect on CH₄ emission or lactational performance of the cows in Exp. 3. Overall, AT included at 0.50% in the ration of dairy cows can have a large mitigation effect on enteric CH₄ emission, but dry matter intake and milk production may also decrease. There was a marked decrease in the CH₄ mitigation potential of AT in the second half of Exp. 3, likely resulting from CHBr₃ decay over time.     

Serum leptin levels in normal children: relationship to age, gender, body mass index, pituitary-gonadal hormones, and pubertal stage

It is commonly accepted that at least in girls puberty starts when a minimum level of body mass or a certain amount of body fat are present. However the precise signal by which adipose stores inform the hypothalamus of the degree of energetic reserves is unknown. Leptin is a hormone produced by the adipocytes to regulate food intake and energy expenditure at the hypothalamic level. To understand whether leptin is the adipose tissue signal that allows puberty, 789 normal children of both sexes, age 5-15 yr, were transversally studied. Leptin levels, as well as gonadal and gonadotropins, levels, were analyzed in addition to the determination of auxological parameters. In an age-related analysis, leptin levels in girls rose from 5-15 yr (from 4.3 +/- 0.4 to 8.5 +/- 0.9 micrograms/L) in parallel with body weight. Boys always had lower leptin levels than girls (3.3 +/- 0.3 micrograms/L at 5 yr), but they rose in parallel with weight until 10 yr (5.3 +/- 0.7 micrograms/L), when a striking decrease was observed until 15 yr (3.0 +/- 0.3 micrograms/L). In girls, leptin was the first hormone to rise followed by FSH and later by LH and estradiol. A similar pattern occurred in boys, despite the fact that leptin dropped after 10 yr when testosterone rises. Divided into three pubertal stages, i.e. P1 = prepuberty, P2 = early puberty, and P3 = overt puberty, in girls the four hormones rose progressively from P1 to P3, but from P2 to P3 the present increment was greater for LH and estradiol. In boys, leptin decreased from P1 to P3, whereas FSH, LH, and testosterone rose. The age-related changes were not caused by adiposity variations, because data did not change when subtracting values of children over 97% of standard deviation score of body mass index. In conclusion: 1) leptin appears to increase in both boys and girls before the appearance of other reproductive hormones related to puberty; 2) leptin levels in boys are always lower than in girls, although they increase with age until the age 10 yr; 3) leptin in boys declines about the time testosterone increases. Leptin may well be a permissive factor for the initiation of pubertal events.     

Enrichment of benzo[a]pyrene in smoked food products and determination by high-performance liquid chromatography-fluorescence detection

We developed a procedure for trace enrichment of benzo[a]pyrene (BP) in extracts of smoked food products, and an HPLC-fluorescence detection (FL) method for determination of BP in the enriched extracts. The procedure consists in extraction/sonication of the lyophilized product in hexane, clean-up of the hexane extract by passage through a Sep-Pak Silica Plus cartridge and, subsequently, by partitioning between hexane and dimethyl sulphoxide, and concentration of the BP using a Sep-Pak C18 Plus cartridge. HPLC-FL and quantification limits were 0.049 microgram/l in acetonitrile (< 0.0067 microgram/kg of smoked food) and 0.089 microgram/l in acetonitrile (< 0.012 microgram/kg), respectively. Recovery (94.1%) and RSD (< 8.65%) were satisfactory. When applied to 15 types of sausage, mean BP content was 0.022 microgram/kg, and all but two samples (both treated with wood smoke) had BP contents below the 0.03 microgram/kg limit imposed in EU legislation for smoking-flavour agents.     

Amyrel, a paralogous gene of the amylase gene family in Drosophila melanogaster and the Sophophora subgenus

We describe a gene from Drosophila melanogaster related to the alpha-amylase gene Amy. This gene, which exists as a single copy, was named Amyrel. It is strikingly divergent from Amy because the amino acid divergence is 40%. The coding sequence is interrupted by a short intron at position 655, which is unusual in amylase genes. Amyrel has also been cloned in Drosophila ananassae, Drosophila pseudoobscura, and Drosophila subobscura and is likely to be present throughout the Sophophora subgenus, but, to our knowledge, it has not been detected outside. Unexpectedly, there is a strong conservation of 5' and 3' flanking regions between Amyrel genes from different species, which is not the case for Amy and which suggests that selection acts on these regions. In contrast to the Amy genes, Amyrel is transcribed in larvae of D. melanogaster but not in adults. However, the protein has not been detected yet. Amyrel evolves about twice as fast as Amy in the several species studied. We suggest that this gene could result from a duplication of Amy followed by accelerated and selected divergence toward a new adaptation.